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Tentative Lecture Topics:

 

Date

Topic
Reading Assignment Instructor

Mon Aug 29

Introduction

History of Agricultural Advances

Printable Version (Word)

Lecture Handout (pdf)

 

J. Knight. 2003. A dying breed. Nature. 421:568-570

King

Wed Aug 31

Tissue culture applications; Micropropagation: preservation & variation

 Lecture Handout

D.C.W. Brown, T.A. Thorpe. 1995. Crop improvement through tissue culture. World Journal of Microbiology and Biotechnology. 11(4):409-415

King

Fri Sept

2

Mutation breeding

Definitions handout

Miller, Waskom, Brick & Chapman. 1991. Transferring in vitro technology to the field. Bio/Technology. 9:143-146.

King

Mon Sept

5

Special breeding considerations: Embryo culture & haploids

King

Wed Sept

7

In vitro hybridization, protoplast fusion

Earle & Sigareva. 1997. Direct transfer of a cold-tolerant ogura male-sterile cytoplasm into cabbage (Brassica oleracea ssp. Capitata) via protoplast fusion. Theor Appl Genet. 94:213-220.

King

Fri Sept

9

Exam 1: 10% of grade

 

King

Mon Sept 12

Applications of Molecular Markers in Breeding

Thro et al. 2004. Genomics and Plant breeding. Crop Sci. 44:1893-1919

Menz

Wed Sept 14

RFLP, SSR, AFLP, and SNP

 Schlotterer.2004. The evolution of mol. Markers. Nature Reviews Genetics 5:63-69

Menz

Fri Sept 16

Construction of a genetic map

 

Menz

Mon Sept 19

Segregation, Linkage, Recombination

 

Menz

Wed Sept 21

Mapping major Genes

 

Menz

Fri Sept 23

MapMaker/ Exp Exercise (Computer Lab)

 

Menz

Mon Sept 26

QTL analysis:  Genetic Models

 

Menz

Wed Sept 28

QTL analysis: Single Marker Analysis

 

Menz

Fri Sept 30

QTL Analysis: Interval mapping

 

Menz

Mon Oct

3

QTL analysis: Composite Interval mapping

 

Menz

Wed

Oct

5

Mapmaker/QTL exercise (Computer lab)

 

Menz

Fri

Oct

7

QTL Cartographer exercise (Computer lab)

 

Menz

Mon Oct

10

Limitations of QTL analysis. QTL analysis assignment (10% grade)

 

Menz

Wed Oct

12

Fingerprinting and Haplotyping

 

Menz

Fri

Oct

14

Associative Genetics

 

Menz

Mon Oct

17

Genetic Diversity

 

Menz

Wed Oct

19

Marker Assisted Selection

 

Menz

Fri

Oct

21

Gene Pyramiding

 

Menz

Mon Oct

24

Marker Assisted Backcrosses

 

Menz

Wed Oct

26

Theoretical and Empirical Results

Ashikari et al. 2005. Cyt okinin Oxidase regulates rice grain production. Science 309:741-745

Menz

Fri

Oct

28

Exam 2: 30% of Grade

 

Menz

Mon Oct

31

Genetic Engineering Introduction: Recombinant DNA Technology

King

Wed

Nov

2

Genetic Engineering Introduction: Recombinant DNA Technology

Michael Schroda, Christoph F. Beck and Olivier Vallon. 2002. Sequence elements within an HSP70 promoter counteract transcriptional transgene silencing in Chlamydomonas. The Plant Journal. 31(4): 445-455.

King

Fri Nov

4

Overview of "Biotech Breeding"

King

Mon Nov

7

Overview of Biotech Breeding

 (Discussion)

King

Wed Nov

9

Overview of Biotech Breeding

 

King

Fri Nov 11

Dr. King out of Town (Possible Guest Lecture)

 

King

Mon Nov 14

Overview of Biotech Breeding: Specific Traits

 

King

Wed Nov 16

Overview of Biotech Breeding: Specific Traits

 

King

Fri Nov 18

Overview of Biotech Breeding: Specific Traits

King

Mon Nov 21

Overview of Biotech Breeding: Specific Traits

King

Wed Nov 23

 

King

Mon Nov 28

Regulatory

USDA-APHIS Regulations: http://www.aphis.usda.gov/brs/7cfr340.html

Regulatory Handouts

King

Wed Nov 30

Regulatory

Germplasm Selection

 

King

Fri Dec

2

Exam 3: 30% of grade

 

King

Mon Dec

5

Review for Final

 

King/Menz

Mon Dec 12

Comprehensive Final: 20% of grade

 

King/Menz

 

Useful Links for Genetic Engineering Section:

USDA APHIS Biotechnology Home Page:

http://www.aphis.usda.gov/brs/index.html